Coding

Part:BBa_K209409:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI C-D part, Tuba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 226
    Illegal EcoRI site found at 809
    Illegal EcoRI site found at 2820
    Illegal PstI site found at 302
    Illegal PstI site found at 313
    Illegal PstI site found at 2437
    Illegal PstI site found at 3457
    Illegal PstI site found at 3610
    Illegal PstI site found at 3820
    Illegal PstI site found at 4056
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 226
    Illegal EcoRI site found at 809
    Illegal EcoRI site found at 2820
    Illegal PstI site found at 302
    Illegal PstI site found at 313
    Illegal PstI site found at 2437
    Illegal PstI site found at 3457
    Illegal PstI site found at 3610
    Illegal PstI site found at 3820
    Illegal PstI site found at 4056
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 226
    Illegal EcoRI site found at 809
    Illegal EcoRI site found at 2820
    Illegal BglII site found at 2721
    Illegal BglII site found at 2972
    Illegal BamHI site found at 10
    Illegal BamHI site found at 2087
    Illegal BamHI site found at 4631
    Illegal XhoI site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 226
    Illegal EcoRI site found at 809
    Illegal EcoRI site found at 2820
    Illegal PstI site found at 302
    Illegal PstI site found at 313
    Illegal PstI site found at 2437
    Illegal PstI site found at 3457
    Illegal PstI site found at 3610
    Illegal PstI site found at 3820
    Illegal PstI site found at 4056
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 226
    Illegal EcoRI site found at 809
    Illegal EcoRI site found at 2820
    Illegal PstI site found at 302
    Illegal PstI site found at 313
    Illegal PstI site found at 2437
    Illegal PstI site found at 3457
    Illegal PstI site found at 3610
    Illegal PstI site found at 3820
    Illegal PstI site found at 4056
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 269
    Illegal BsaI.rc site found at 509
    Illegal BsaI.rc site found at 1557
    Illegal BsaI.rc site found at 3134
    Illegal SapI site found at 3279
    Illegal SapI site found at 3873
    Illegal SapI site found at 4307
    Illegal SapI.rc site found at 1266
    Illegal SapI.rc site found at 2043
    Illegal SapI.rc site found at 2134
    Illegal SapI.rc site found at 3829


Design Notes

Cloned into pCR2.1.

Source

References